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SEC-MALLS

Size Exclusion Chromatography - Multi Angle Laser Light Scattering

Application

By providing an absolute measure of weight average molar mass (Mw) (± 5%) in less than 20 minutes, SEC-MALLS is the method of choice for fast and accurate determination of molecular mass and related properties, like protein oligomerisation state and homogeneity, including protein modifier content like detergent micelles or large polysaccharides. With an additional built-in DLS module that can operate both inline and from cuvettes, it is possible to calculate hydrodynamic radius (Rh) from the same experiment.

Equipment

HPLC equipped with UV/VIS and fluorescent detectors (Shimadzu).
Other detectors connected online: Dawn8+, QELS, Optilab T-rEX (Wyatt).

Location

Astbury 6.110

Contact

Maria Nikolova
School of Biomedical Sciences
Email: bsmni@leeds.ac.uk