Atomic Force Microscopy (AFM)
Application
Imaging down to 1 nm lateral and 0.05 nm vertical resolution of any surface in any environment. Specialising in the dynamics of soft-matter/biological samples in an aqueous environment. We can also image in humidity controlled gas, organic solvents, acids and bases. Temperature control from 1 - 120 °C (gas) and 1-45 °C (liquid). High resolution dynamic imaging at rates of 1 frame per sec in liquids. Peak Force QNM mode reduces imaging force to <50pN and allows access to full mechanical characterisation at the nanoscale, such as modulus, adhesion and deformation. For correlative optical microscopy, the JPK Nanowizard 4 AFM is combined with an inverted scanning confocal microscope with Fluorescence Lifetime capability, and the MFP-3D-BIO is positioned on an inverted epifluorescence microscope. Many AFM modes are available, such as Variable Field Magnetic Force, Surface Potential/Kelvin Probe and Surface Conduction.
Equipment
- FastScan-Bio AFM (Bruker) – high speed imaging
- JPK Nanowizard 4 AFM (Bruker) / PicoQuant Microtime 200 FLIM Confocal Microscope
- Icon AFM (Bruker) – large sample, large scan range
- 2 x Multimode 8 (Nanoscope V) AFM (Bruker) – high resolution + mechanics
- MFP-3D-Bio AFM (Asylum Research) – combined optical system
- MFP-3D AFM (Asylum Research) - single molecule protein force puller
- Dimensions 3100 Nanoman AFM (Bruker) – MFM, electric potential
- VT-STM (ScientaOmicron) High Vacuum AFM/STM with T-range 100-600 K LT-STM/NC-AFM
Contact
Dr Simon Connell
School of Physics and Astronomy
Email: S.D.A.Connell@leeds.ac.uk
Tel: 0113 343 8241